가토의 골수 세포에서 분화된 골모세포의 골 형성에 혈소판 농축 혈장이 미치는 효과: 조직 형태학적 분석
Effect of Platelet-Rich Plasma on Osteogenesis of Marrow-Derived Osteoblasts in The Mandible of Rabbit: Histomorphometric Analysis
박영주, 임성철, 강태인, 정재안, 신진업, 전민수, 김보균, 송준호, 연병무,
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박영주 ( ) - 한림대학교의료원 강남성심병원 구강악안면외과학교실
임성철 ( Lim Sung-Chul ) - 조선대학교 의과대학 병리학교실
강태인 ( Gang Tae-In ) - 한림대학교 의과대학 치과학교실
정재안 ( Chung Jae-An ) - 한림대학교 강남성심병원 구강악안면외과
신진업 ( Shin Jin-Eob ) - 한림대학교 강남성심병원 구강악안면외과
전민수 ( Jeon Min-Su ) - 한림대학교의료원 강남성심병원 구강악안면외과학교실
김보균 ( Kim Bo-Kyun ) - 한림대학교의료원 강남성심병원 구강악안면외과학교실
송준호 ( Song Jun-Ho ) - 한림대학교의료원 강남성심병원 구강악안면외과학교실
연병무 ( Yeon Byoung-Moo ) - 한림대학교의료원 강남성심병원 구강악안면외과학교실
KMID : 0360120070290060474
Abstract
Purpose: The effect of platelet-rich plasma(PRP) on osteogenesis of marrow-derived osteoblasts on histomorphometric analysis in the mandible of rabbit was assessed.
Material and Methods : Bone marrow cells were obtained from iliac bone of rabbits and were cultured in a Dulbecco’s Modified Eagle’s Medium(DMEM) with Dexamethasone, L-Ascortic acid, beta-Glycerophosphate to proliferate and differentiate into osteoblasts for 4~5 weeks. The expression of osteogenic mar-kers was detected by reverse transcription-polymerase chain reaction(RT-PCR) and silver nitrate stain. Then we prepared bony defects in the mandible of rabbit, 10.0mm in diameter and 4.0mm deep, by trephine bur. In the control group, the defects were filled with autogenous bone and cultured osteoblasts. In the experimental group, the defects were filled with autogenous bone, cultured osteoblasts and PRP. 2 weeks, 4 weeks, 8 weeks later, each group was evaluated with histological and histomorphometric analyses.
Results: In vitro, osteoblasts were identified on RT-PCR and silver nitrate stain. According to histological observation, at 2 weeks well-developed anasto-mosing newly-formed woven bone was observed, at 4 weeks anastomosing newly-formed woven bone having osteoblastic activation was observed, and at 8 weeks thick newly-formed woven bone was observed in both control and experimental groups. According to histomorphometric analysis, there were 1.5% more newly-formed bone volume in experimental group than control group at 2 weeks, 28.4% more at 4 weeks, 4.3% more at 8 weeks. Particularly there were significant differences in bone volume at 4 weeks and 8 weeks new bone.
Conclusion: Our results demonstrated PRP may enhance osteogenesis of marrow-derived osteoblasts at 4 weeks, 8 weeks.
키워드
Osteoblasts; Bone marrow cell; Platelet-rich plasma; PRP; Osteogenesis
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